Protein Purification Reagents and Kits
Biotage IMAC PHYTIP COLUMNS 1ML
IMAC PhyTip columns (1 mL volume) containing 20 ul affinity media per column, Tecan. Incl. buffer kit
STEMCELL Technologies RoboSep™ Mouse CD4+CD62L+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119). Then CD4+CD62L+ cells, pre-labeled with CD62L PE, are selected using the EasySep™ PE Selection Cocktail. The desired cells are labeled with antibodies and magnetic particles. The cells are separated without columns using an EasySep™ magnet.For the isolation of mouse naïve CD4+ T cells in as little as 15 minutes by negative selection, we recommend using the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit (19765).
STEMCELL Technologies EasySep Human EGFR Positive S
Immunomagnetic positive selection of human EGFR+ cells
American Research Products Inc Mouse Lymphocyte actiVation ge
Mouse Lymphocyte actiVation gene 3 protein (LAG3) ELISA kit from CUSABIO Detection Range 1 25 ng/mL-80 ng/mL Sensitivity 0 31 ng/mL Sample serum plasma tissue homogenates Method Sandwich
iQ Biosciences SD Rat Splenocytes, 50M cells/vial
High-quality, high-viability (typically > 70%) splenocytes from the Sprague Dawley rat, a commonly-used outbred rat strain. Used for a wide variety of immunology-based applications, including in-vitro immunoassays and ex-vivo applications. All orders come with an iQ Certificate of Analysis.
Biotium CD45RA(111-1C5), CF488A conjugate, 0.1mg/mL
Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO positive) from B cell lymphomas (CD45RA positive).Primary antibodies are available purified, or with a selection of fluoresc
STEMCELL Technologies RoboSep™ HLA Total Lymphocyte Enrichment: Complete Processing Kit for Whole Blood with Filter Tips, For processing: 200 mL whole blood processed
The EasySep™ Total Lymphocyte Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human lymphocytes from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD14, CD15, CD16, CD33, CD36, CD41, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are bound to magnetic particles and separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Mouse APC Positive Selection Kit II, For processing: 1 x 10^9 cells
The EasySep™ Mouse APC Positive Selection Kit II is designed to isolate cells that are labeled with APC (allophycocyanin)-conjugated antibodies. Desired cells are targeted with antibody complexes recognizing APC and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse APC Positive Selection Kit (Catalog #18452).
Biotium Primary Antibody CD8a SK1 CF514 1/EA
CD8a (clone SK1) is a mouse monoclonal antibody that recognizes the human protein CD8 CD8a is a common marker of cytotoxic (Killer) T cells and is a T cell coreceptor This CF514 antibody conjugate has been validated in flow cytometry
STEMCELL Technologies EasySep™ Direct Human PBMC Isolation Kit RoboSep™, For processing: 100 mL whole blood processed
The EasySep™ Direct Human PBMC Isolation Kit is designed to isolate highly purified peripheral blood mononuclear cells (PBMCs) from fresh whole blood, buffy coat, bone marrow, cord blood, or leukapheresis products by negative selection. Red blood cells (RBCs), platelets, and unwanted cells are targeted for removal with antibody complexes and magnetic particles and separated using an EasySep™ magnet. Untouched PBMCs are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction.
STEMCELL Technologies RoboSep™ Human CD8+ T Cell Enrichment Kit with Filter Tips, For processing: 1 x 10^9 cells
The EasySep™ Human CD8+ T Cell Enrichment Kit is designed to isolate CD8+ T cells from fresh or previously frozen peripheral blood mononuclear cells or ammonium chloride-lysed leukapheresis sample by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-CD8+ T cells and glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.For even faster cell isolations, we recommend the new EasySep™ Human CD8+ T Cell Isolation Kit (17953) (http://www.stemcell.com/en/Products/All-Products/EasySep-Human-CD8-T-Cell-Isolation-Kit.aspx) which isolates cells in just 8 minutes.
STEMCELL Technologies EasySep™ Mouse CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-CD8+ T cells and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.This product replaces the EasySep™ Mouse CD8+ T Cell Enrichment Kit (Catalog #19753) for even faster cell isolations.
STEMCELL Technologies RosetteSep™ HLA T Cell Enrichment Cocktail, For processing: 1000 mL whole blood processed
Immunodensity isolation of untouched T cells for HLA analysis
STEMCELL Technologies RoboSep™ HLA B Cell Enrichment: Complete Processing Kit for Whole Blood with Filter Tips, For processing: 200 mL whole blood processed
The EasySep™ HLA B Cell Enrichment Kit: Complete Processing Kit for Whole Blood is designed to isolate human B cells from HetaSep™-treated whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD3, CD14, CD16, CD33, CD36, CD41, CD43, CD56, CD66b, glycophorin A and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
STEMCELL Technologies EasySep™ Direct HLA Crossmatch B Cell Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct HLA Crossmatch B Cell Isolation Kit can be used to isolate highly purified B cells directly from human whole blood, spleen, or lymph nodes by immunomagnetic negative selection. This cell isolation kit targets non-B cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and and are immediately available for downstream applications such as flow cytometry crossmatch.